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Goat anti-POU2AF1 Antibody
Item#:
dAP-2414
Size Price Quantity
50.0 ug $310.00
Goat anti-POU2AF1 Antibody
Principle Name POU2AF1
Official Symbol POU2AF1
All Name and Symbols POU2AF1; POU class 2 associating factor 1; BOB1; OBF-1; OBF1; OCAB; POU domain, class 2, associating factor 1
Accession Number(s) NP_006226.2
Peptide Sequence DSDAYALNHTLSVE, is from C Terminus
Human Gene ID(s) 5450
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB Trf, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag
Immunohistochemistry In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5µg/ml.
Reference(s) Duan H, Xiang H, Ma L, Boxer LM, Functional long-range interactions of the IgH 3' enhancers with the bcl-2 promoter region in t(14;18) lymphoma cells. Oncogene 2008 Dec 27 (53)

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