Goat anti-IDH3A Antibody |
| Principle Name | IDH3A |
| Official Symbol | IDH3A |
| All Name and Symbols | IDH3A; isocitrate dehydrogenase 3 (NAD+) alpha; H-IDH alpha; NAD(+)-specific ICDH subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subuni |
| Accession Number(s) | NP_005521.1 |
| Peptide Sequence | DFTEEICRRVKDLD, is from C Terminus |
| Human Gene ID(s) | 3419 |
| Non-Human GeneID(s) | 67834 (mouse); 114096 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat, Pig |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1). Recommen |
| Reference(s) | Bzymek KP, Colman RF. Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase. Biochemistry. 2007 May 8;46(18) |
