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Goat anti-POU5F1B Antibody
Item#:
dAP-2111
Size Price Quantity
50.0 ug $310.00
Goat anti-POU5F1B Antibody
Principle Name POU5F1B
Official Symbol POU5F1B
All Name and Symbols POU5F1B; POU class 5 homeobox 1B; OCT4-pg1; OTF3C; OTF3P1; POU5F1P1; POU5FLC20; POU5FLC8; POU 5 domain protein; POU class 5 homeobox 1 pseudogene 1; POU domain transcription factor Oct-4; POU domain, class 5, transcription factor 1 pseudogene 1; octamer b
Accession Number(s) NP_001153014.1
Peptide Sequence TPGYGSPHFTALYS, is from internal region (near C Terminus)
Human Gene ID(s) 5462; 5460
Non-Human GeneID(s)
Important Comments This antibody is expected to crossreact to the almost identical OCT4 / POU5F1 (GeneID 5460).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6kDa
Reference(s) Panagopoulos I, Möller E, Collin A, Mertens F, The POU5F1P1 pseudogene encodes a putative protein similar to POU5F1 isoform 1. Oncology reports 2008 Nov 20 (5)

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