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Goat anti-DIAPH1 Antibody


Item#:
dAP-2020
Size Price Quantity
50.0 ug $310.00
Goat anti-DIAPH1 Antibody
Principle Name DIAPH1
Official Symbol DIAPH1
All Name and Symbols DIAPH1; diaphanous homolog 1 (Drosophila); DFNA1; DIA1; DRF1; FLJ25265; LFHL1; hDIA1; diaphanous 1; diaphanous-1; diaphanous-related formin 1
Accession Number(s) NP_005210.3; NP_001073280.1
Peptide Sequence QTKTSKAKKDQ, is from internal region
Human Gene ID(s) 1729
Non-Human GeneID(s) 13367 (mouse);
Important Comments This antibody is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Mouse
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 140kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141kDa according to Human NP_005210.3 and 139kDa according to Mouse NP_031884.1). Recommended concentration
Reference(s) Higashi T, Ikeda T, Shirakawa R, Kondo H, Kawato M, Horiguchi M, Okuda T, Okawa K, Fukai S, Nureki O, Kita T, Horiuchi H Biochemical characterization of the Rho GTPase-regulated actin assembly by diaphanous-related formins, mDia1 and Daam1, in platelets J Biol Chem. 2008 Mar 28;283(13)

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