Goat anti-Calponin 2 / CNN2 Antibody

Item#:

dAP-1852

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Calponin 2 / CNN2 Antibody
Principle Name	Calponin 2 / CNN2
Official Symbol	CNN2
All Name and Symbols	CNN2; calponin 2; calponin H2, smooth muscle; neutral calponin
Accession Number(s)	NP_004359.1; NP_958434.1
Peptide Sequence	DPGEVPEYPPYYQEE, is from internal region (near C Terminus)
Human Gene ID(s)	1265
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 33.7kDa according to NP_004359.1). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Placenta. Recommended concentration
Reference(s)	Masuda H, Tanaka K, Takagi M, Ohgami K, Sakamaki T, Shibata N, Takahashi K, Molecular cloning and characterization of human non-smooth muscle calponin. Journal of biochemistry 1996 Aug 120 (2)