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Goat anti-CTLA + CTLB Antibody


Item#:
dAP-1611
Size Price Quantity
50.0 ug $310.00
Goat anti-CTLA + CTLB Antibody
Principle Name CTLA + CTLB
Official Symbol CLTA
All Name and Symbols CLTA; CTLB; clathrin, light chain (Lca); LCA; clathrin, light polypeptide (Lca); clathrin, light polypeptide A; clathrin, light chain (Lcb); LCB
Accession Number(s) NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1
Peptide Sequence CDFNPKSSKQAKD, is from internal region
Human Gene ID(s) 1211; 1212
Non-Human GeneID(s) 12757 (mouse); 83800 (rat);
Important Comments This antibody is expected to recognize all reported isoforms of light chains a and b.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different anti
Immunohistochemistry Paraffin embedded Human Spleen. Recommended concentration
Reference(s) Poupon V, Girard M, Legendre-Guillemin V, Thomas S, Bourbonniere L, Philie J, Bright NA, McPherson PS. Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly. Proc Natl Acad Sci U S A. 2008 Jan 8;105(1)

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