Goat anti-CHRNA4 Antibody

Item#:

dAP-1362

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CHRNA4 Antibody
Principle Name	CHRNA4
Official Symbol	CHRNA4
All Name and Symbols	CHRNA4; cholinergic receptor, nicotinic, alpha 4; BFNC; EBN; EBN1; NACRA4; cholinergic receptor, nicotinic, alpha 4 subunit; cholinergic receptor, nicotinic, alpha polypeptide 4; neuronal acetylcholine receptor alpha 4 subunit; neuronal nicotinic acetylch
Accession Number(s)	NP_000735.1
Peptide Sequence	RASSHVETRAHAE, is from internal region (near the N Terminus)
Human Gene ID(s)	1137
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Preliminary experiments in Human Brain (Cerebral Cortex, Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field -
Reference(s)	Bertrand D, Picard F, Le Hellard S, Weiland S, Favre I, Phillips H, Bertrand S, Berkovic SF, Malafosse A, Mulley J. How mutations in the nAChRs can cause ADNFLE epilepsy. Epilepsia. 2002;43 Suppl 5