Goat anti-Pyruvate Carboxylase Antibody

Item#:

dAP-1316

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Pyruvate Carboxylase Antibody
Principle Name	Pyruvate Carboxylase
Official Symbol	PC
All Name and Symbols	PC; pyruvate carboxylase; PCB
Accession Number(s)	NP_000911.2; NP_001035806.1; NP_071504.2
Peptide Sequence	KFKEVKKAYVEANQ, is from internal region
Human Gene ID(s)	5091
Non-Human GeneID(s)	18563 (mouse); 25104 (rat);
Important Comments	All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 150kDa band observed in Mouse Liver lysates (calculated MW of 130kDa according to NP_000911.2, NP_001035806.1 and NP_071504.2). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Liver. Recommended concentration
Reference(s)	St Maurice M, Reinhardt L, Surinya KH, Attwood PV, Wallace JC, Cleland WW, Rayment I. Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme. Science. 2007 Aug 24;317(5841)