Goat anti-PCBP4 Antibody |
| Principle Name | PCBP4 |
| Official Symbol | PCBP4 |
| All Name and Symbols | PCBP4; poly(rC) binding protein 4 ; LIP4; MCG10 ; LYST-interacting protein; RNA binding protein MCG10; alphaCP-4 protein; poly(rC)-binding protein 4; alpha CP-4; |
| Accession Number(s) | NP_065151.1; NP_127501.1; NP_127502.1; NP_127503.1 |
| Peptide Sequence | NGSKKAERQKFSPY, is from C Terminus |
| Human Gene ID(s) | 57060 |
| Non-Human GeneID(s) | 59092 (mouse); 363133 (rat); |
| Important Comments | This antibody is expected to recognise all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_127503.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx. 40kDa band observed in lysates of cell line HeLa (calculated MW of 41.4kDa according to NP_127502.1 and NP_127503.1). In transfected HEK293 transiently expressing PCBP4 a band of approx. 45kDa is observed. This band is not observed |
| Reference(s) | Pio R, Zudaire I, Pino I, Castano Z, Zabalegui N, Vicent S, Garcia-Amigot F, Odero MD, Lozano MD, Garcia-Foncillas J, Calasanz MJ, Montuenga LM. Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant alpha CP-4a, is underexpressed in lung cancer. Cancer Res. 2004 Jun 15;64(12) |
