Goat anti-NONO / p54NRB Antibody

Item#:

dAP-0852

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-NONO / p54NRB Antibody
Principle Name	NONO / p54NRB
Official Symbol	NONO
All Name and Symbols	NONO; P54NRB ; non-POU domain containing, octamer-binding ; HGNC
Accession Number(s)	NP_031389.3
Peptide Sequence	NRAAPGAEFAPNK, is from C Terminus
Human Gene ID(s)	4841
Non-Human GeneID(s)	53610 (mouse); 317259 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 60kDa band observed in Mouse Brain lysates (calculated MW of 54.2kDa according to Human NP_031389.3 and 54.5kDa according to Mouse NP_075633.2). In transfected HEK293 transiently expressing Human NONO a band of approx. 55kDa is obser
Immunohistochemistry	In paraffin embedded Human Spleen shows nuclear staining in select splenocytes. Recommended concentration
Reference(s)	Brown SA, Ripperger J, Kadener S, Fleury-Olela F, Vilbois F, Rosbash M, Schibler U. PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. Science. 2005 Apr 29;308(5722)