Goat anti-GEF5 Antibody |
| Principle Name | GEF5 |
| Official Symbol | ARHGEF5 |
| All Name and Symbols | GEF5; DKFZp686N1969; ARHGEF5; Rho guanine nucleotide exchange factor (GEF) 5; P60; TIM; TIM1; guanine nucleotide regulatory protein TIM; oncogene TIM rho guanine nucleotide exchange factor 5; transforming immortalized mammary oncogene |
| Accession Number(s) | NP_005426.2 |
| Peptide Sequence | DKEIDQNSQQEE, is from internal region |
| Human Gene ID(s) | 7984 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Placenta. Recommended concentration |
| Reference(s) | Debily MA, Camarca A, Ciullo M, Mayer C, El Marhomy S, Ba I, Jalil A, Anzisi A, Guardiola J, Piatier-Tonneau D. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer. Hum Mol Genet. 2004 Feb 1;13(3) |
