Goat anti-IFT88 / Polaris Antibody

Item#:

dAP-0771

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-IFT88 / Polaris Antibody
Principle Name	IFT88 / Polaris
Official Symbol	IFT88
All Name and Symbols	IFT88; polaris; TTC10; tetratricopeptide repeat domain 10; HGNC
Accession Number(s)	NP_783195.2; NP_006522.2
Peptide Sequence	KKRIDEDDFADEE, is from C Terminus
Human Gene ID(s)	8100
Non-Human GeneID(s)	21821 (mouse); 305918 (rat);
Important Comments	This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2)
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC, IF
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments in Human Kidney, Liver and Lung lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
Immunohistochemistry	In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration
Reference(s)	Vogel G. News focus