Goat anti-ACHE Antibody |
| Principle Name | ACHE |
| Official Symbol | ACHE |
| All Name and Symbols | ACHE; YT; acetylcholinesterase (YT blood group); ARACHE; N-ACHE; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase |
| Accession Number(s) | NP_000656.1; NP_001269378.1 |
| Peptide Sequence | QFDHYSKQDRCSDL, is from C Terminus |
| Human Gene ID(s) | 43 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IF, FC |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 65-70kDa band observed in lysates of cell lines Daudi, HeLa and Jurkat, and approx. 70kDa in Rat Brain lysates (calculated MW of 67.8kDa according to Human NP_000656.1 and 68.2kDa according to Rat NP_742006.1). Recommended concentra |
| Reference(s) | Cottingham MG, Voskuil JL, Vaux DJ. The intact human acetylcholinesterase C-terminal oligomerization domain is alpha-helical in situ and in isolation, but a shorter fragment forms beta-sheet-rich amyloid fibrils and protofibrillar oligomers. Biochemistry. 2003 Sep 16;42(36) |
