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Goat anti-RIF1 (aa 2076-2089) Antibody
Item#:
dAP-0585
Size Price Quantity
50.0 ug $310.00
Goat anti-RIF1 (aa 2076-2089) Antibody
Principle Name RIF1 (aa 2076-2089)
Official Symbol RIF1
All Name and Symbols Rif1; telomere-associated protein RIF1 homolog; Rap1 interacting factor 1; RAP1 interacting factor homolog (yeast); DKFZp434D1026; DKFZp781N1478; FLJ12870; OTTHUMP00000162712; RAP1 interacting factor 1
Accession Number(s) NP_060621.3
Peptide Sequence CEEGIIDANKTETNT, is from internal region
Human Gene ID(s) 55183
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise an epitope corresponding to aa 2076-2089 of human RIF1 protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 247kDa according to NP_060621. Therefore we cannot recommend an optimal concentration.
Immunohistochemistry Paraffin embedded Human Testis and Brain (Cortex). Recommended concentration
Reference(s) Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T. Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev. 2004 Sep 1;18(17)

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