Goat anti-Rabenosyn 5 Antibody

Item#:

dAP-0504

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Rabenosyn 5 Antibody
Principle Name	Rabenosyn 5
Official Symbol	ZFYVE20
All Name and Symbols	ZFYVE20; Rabenosyn 5; FYVE-finger-containing Rab5 effector protein rabenosyn-5; zinc finger, FYVE domain containing 20; zinc finger, FYVE domain containing 20; FLJ34993; MGC126210; Rabenosyn-5
Accession Number(s)	NP_071735.2
Peptide Sequence	ELKHTLAKQKGGTD, is from C Terminus
Human Gene ID(s)	64145
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 110 kDa band observed in A431, Fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). Recommended concentration
Reference(s)	Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M. Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain. J Cell Biol. 2000 Oct 30;151(3)