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Goat anti-TIF1A / TRIM24 Antibody
Item#:
dAP-0466
Size Price Quantity
50.0 ug $310.00
Goat anti-TIF1A / TRIM24 Antibody
Principle Name TIF1A / TRIM24
Official Symbol TRIM24
All Name and Symbols TRIM24; TIF1; transcriptional intermediary factor 1; PTC6; TF1A; TIF1A; Tif1a; hTIF1; TIF1ALPHA; transcriptional intermediary factor 1 alpha; tripartite motif protein 24; tripartite motif-containing 24; RNF82
Accession Number(s) NP_056989.2; NP_003843.3
Peptide Sequence KRLKSIEERQLLK, is from C Terminus
Human Gene ID(s) 8805
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 120kDa band in human kidney and heart lysates at 1ug/ml, however prominent bands were also seen at 25kDa and 45kDa. Please note that currently we cannot find an explanation in the literature for some o
Immunohistochemistry In paraffin embedded Human Breast cancer shows nuclear staining in the distorted lobules. Recommended concentration, 4-6µg/ml.
Reference(s) Thenot S, Henriquet C, Rochefort H, Cavailles V. Differential interaction of nuclear receptors with the putative human transcriptional coactivator hTIF1. J Biol Chem. 1997 May 2;272(18)

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