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Goat anti-PAX3 Antibody


Item#:
dAP-0413
Size Price Quantity
50.0 ug $310.00
Goat anti-PAX3 Antibody
Principle Name PAX3
Official Symbol PAX3
All Name and Symbols PAX3; WS1; CDHS; HUP2; paired box gene 3 (Waardenburg syndrome 1); paired domain gene 3; paired box homeotic gene 3 (Waardenburg syndrome 1); paired box 3; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box homeotic gene 3; paired domain ge
Accession Number(s) NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1
Peptide Sequence TTLAGAVPRMM, is from N Terminus
Human Gene ID(s) 5077
Non-Human GeneID(s) 18505 (mouse); 114502 (rat);
Important Comments This antibody is expected to recognize all 8 reported isoforms (NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 45kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2kDa according to NP_852125.1 and of 44.8kDa according to NP_852126.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration
Reference(s) Tsukamoto K, Nakamura Y, Niikawa N. Isolation of two isoforms of the PAX3 gene transcripts and their tissue-specific alternative expression in human adult tissues. Hum Genet. 1994 Mar;93(3)

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