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Goat anti-BAF53A and BAF53B Antibody
Item#:
dAP-0412
Size Price Quantity
50.0 ug $310.00
Goat anti-BAF53A and BAF53B Antibody
Principle Name BAF53A and BAF53B
Official Symbol ACTL6A / ACTL6B
All Name and Symbols BAF53A; BAF53B; ACTL6; BAF53a; BAF53b; actin-like 6A; actin-like 6B; hArpN alpha; actin-related protein; ACTL6A; ACTL6B; 53 kDa BRG1-associated factor B; ARPN-BETA; MGC5382 Arp4; INO80K; BAF complex 53 kDa subunit; BAF53; BRG1-associated factor; INO80 com
Accession Number(s) NP_004292.1; NP_817126.1; NP_057272.1
Peptide Sequence YEEGGKQCVERKCP, is from C Terminus
Human Gene ID(s) 51412; 86
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both BAF53A and BAF53B, which are almost identical. Reported variants represent identical protein (NP_817126.1; NP_829888.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 48kDa band observed in Hela lysates (predicted MW of 49kDa according to NP_057272, NP_004292). Recommended for use at 1-3µg/ml.
Reference(s) Harata M, Mochizuki R, Mizuno S. Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues. Biosci Biotechnol Biochem. 1999 May;63(5)

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