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Goat anti-E2-230K / UBE2O Antibody
Item#:
dAP-0368
Size Price Quantity
50.0 ug $310.00
Goat anti-E2-230K / UBE2O Antibody
Principle Name E2-230K / UBE2O
Official Symbol UBE2O
All Name and Symbols UBE2O; ubiquitin-conjugating enzyme E2O; E2-230K; FLJ12878; KIAA1734
Accession Number(s) NP_071349.3
Peptide Sequence ALLEAGMPECTEDK, is from C Terminus
Human Gene ID(s) 63893
Non-Human GeneID(s) 217342 (mouse);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any resul
Immunohistochemistry In paraffin embedded Human Heart shows textured staining in some myocardial fibres. Recommended concentration, 4-6µg/ml.
Reference(s) Yokota T, Nagai H, Harada H, Mine N, Terada Y, Fujiwara H, Yabe A, Miyazaki K, Emi M. Identification, tissue expression, and chromosomal position of a novel gene encoding human ubiquitin-conjugating enzyme E2-230k. Gene. 2001 Apr 4;267(1)

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