Goat anti-CAPZB Antibody |
| Principle Name | CAPZB |
| Official Symbol | CAPZB |
| All Name and Symbols | CAPZB; capping protein (actin filament) muscle Z-line, beta; CAPB; CAPZ; CAPPB; Cap Z; RP4-657E11.7; MGC104401; MGC129749; MGC129750; Cap Z; F-actin capping protein beta subunit |
| Accession Number(s) | NP_004921.1 |
| Peptide Sequence | NDLVEALKRKQQC, is from C Terminus |
| Human Gene ID(s) | 832 |
| Non-Human GeneID(s) | 12345 (mouse); |
| Important Comments | This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat, Pig |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 30kDa band observed in lysates of cell lines Jurkat, HeLa and NIH-3T3 and approx 28kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 30.6kDa according to Human NP_004921.1 and 31.3kDa according to Mouse NP_00103 |
| Reference(s) | Hart MC, Cooper JA. Vertebrate isoforms of actin capping protein beta have distinct functions In vivo.gelsolin/severin and profilin protein families. J Cell Biol 1999 Dec 13;147(6) |
