Goat anti-EVC2 / Limbin Antibody

Item#:

dAP-0144

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-EVC2 / Limbin Antibody
Principle Name	EVC2 / Limbin
Official Symbol	EVC2
All Name and Symbols	EVC2; LBN; LIMBIN; Ellis van Creveld syndrome 2 (limbin); limbin
Accession Number(s)	NP_667338.3; NP_001159608.1
Peptide Sequence	LNAKKAMRALGMD, is from C Terminus
Human Gene ID(s)	132884
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise isoform 1 (NP_667338.3) and isoform 2 (NP_001159608.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Consistent band observed in 293 and human heart at approx 75kDa with 1 hour incubation of primary at 0.5ug/ml.
Immunohistochemistry	In paraffin embedded Human Placenta shows strong staining of the cytotrophoblasts. Recommended concentration, 2-4µg/ml.
Reference(s)	Takeda H, Takami M, Oguni T, Tsuji T, Yoneda K, Sato H, Ihara N, Itoh T, Kata SR, Mishina Y, Womack JE, Moritomo Y, Sugimoto Y, Kunieda T. Positional cloning of the gene LIMBIN responsible for bovine chondrodysplastic dwarfism. Proc Natl Acad Sci U S A. 2002 Aug 6;99(16)