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Goat anti-ARIH1 / HHARI Antibody


Item#:
dAP-0131
Size Price Quantity
50.0 ug $310.00
Goat anti-ARIH1 / HHARI Antibody
Principle Name ARIH1 / HHARI
Official Symbol ARIH1
All Name and Symbols ARIH1; HHARI; ARI; HARI; UBCH7BP; ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila); ariadne homolog; ariadne, Drosophila, homolog of; DKFZp686O13120; FLJ20329; FLJ93118; ariadne ubiquitin-conjugating enzyme E2 binding prote
Accession Number(s) NP_005735.2
Peptide Sequence HEGYEKDLWEYIED, is from C Terminus
Human Gene ID(s) 25820
Non-Human GeneID(s) 23806 (mouse);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1µg/ml.
Immunohistochemistry Paraffin embedded Human Adrenal Gland. Recommended concentration
Reference(s) Moynihan TP, Ardley HC, Nuber U, Rose SA, Jones PF, Markham AF, Scheffner M, Robinson PA. The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 1999 Oct 22;274(43)

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