Goat anti-ELMO3 Antibody |
| Principle Name | ELMO3 |
| Official Symbol | ELMO3 |
| All Name and Symbols | ELMO3; engulfment and cell motility 3 (ced-12 homolog, C. elegans); CED12; FLJ13824; ced-12 homolog 3; engulfment and cell motility 3; CED-12; ELMO-3 |
| Accession Number(s) | NP_078988.2 |
| Peptide Sequence | TNFNFCYDCSIAEP, is from C Terminus |
| Human Gene ID(s) | 79767 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 75kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 70.0kDa according to NP_ 078988.2). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Prostate shows vesiculate staining in the cytoplasm of secretory cells. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell 2001 Oct 5;107(1) |
