Goat anti-MTR Antibody |
| Principle Name | MTR |
| Official Symbol | MTR |
| All Name and Symbols | MTR; 5-methyltetrahydrofolate-homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase 1; FLJ33168, FLJ43216; FLJ45386; MS; cblG; cobalamin-dependent methionine synthase; methionine synthase; vitamin-B12 dependent methionine |
| Accession Number(s) | NP_000245.2 |
| Peptide Sequence | VEKWLGPILGYDTD, is from C Terminus |
| Human Gene ID(s) | 4548 |
| Non-Human GeneID(s) | 81522 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 150kDa band seen in HeLa and Jurkat cell lysates. Recommended for use at 1-3 µg/ml. |
| Immunohistochemistry | In paraffin embedded Human Adrenal Gland shows cytoplasm staining in the cortex. Recommended concentration |
| Reference(s) | Chen LH, Liu ML, Hwang HY, Chen LS, Korenberg J, Shane B. Human methionine synthase. cDNA cloning, gene localization, and expression. J Biol Chem 1997 Feb 7;272(6) |
