Goat anti-ORC3L Antibody |
| Principle Name | ORC3L |
| Official Symbol | ORC3L |
| All Name and Symbols | ORC3L; origin recognition complex, subunit 3-like (yeast); LAT; ORC3; LATHEO; IMAGE50150; origin recognition complex, subunit 3 (yeast homolog)-like; OTTHUMP00000040611; homolog of latheo, Drosophila; RP3-486L4.1; origin recognition complex, subunit 3 |
| Accession Number(s) | NP_862820.1; NP_036513.2 |
| Peptide Sequence | QKTDHVARLTWGGC, is from C Terminus |
| Human Gene ID(s) | 23595 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 75kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3kDa according to NP_862820.1 and NP_036513.2). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Brain (Cortex). Recommended concentration |
| Reference(s) | Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A. latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant. Neuron 1999 May;23(1) |
