Goat anti-PPP1R15A / GADD34 Antibody

Item#:

dAP-0019

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PPP1R15A / GADD34 Antibody
Principle Name	PPP1R15A / GADD34
Official Symbol	PPP1R15A
All Name and Symbols	PPP1R15A; GADD34; protein phosphatase 1, regulatory (inhibitor) subunit 15A; growth arrest and DNA-damage-inducible 34; protein phosphatase 1, regulatory subunit 15A
Accession Number(s)	NP_055145.3
Peptide Sequence	AAALDLSGRRG, is from C Terminus
Human Gene ID(s)	23645
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC, IF, FC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 75kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration
Reference(s)	Kojima E, Takeuchi A, Haneda M, Yagi A, Hasegawa T, Yamaki K, Takeda K, Akira S, Shimokata K, Isobe K. The function of GADD34 is a recovery from a shutoff of protein synthesis induced by ER stress