Goat anti-PRAM1 Antibody |
| Principle Name | PRAM1 |
| Official Symbol | PRAM1 |
| All Name and Symbols | PRAM-1; PRAM-1 protein; PML-RARA target gene encoding an Adaptor Molecule-1; PML-RARA regulated adaptor molecule 1; MGC39864; PML-RAR |
| Accession Number(s) | NP_115528.4 |
| Peptide Sequence | DFCDPLENQPLPLGR, is from C Terminus |
| Human Gene ID(s) | 84106 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3µg/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, J |
| Reference(s) | Moog-Lutz C, Peterson EJ, Lutz PG, Eliason S, Cave-Riant F, Singer A, Di Gioia Y, Dmowski S, Kamens J, Cayre YE, Koretzky G. PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells. J Biol Chem 2001 Jun 22;276(25) |
