Goat anti-ATF7 Antibody |
| Principle Name | ATF7 |
| Official Symbol | ATF7 |
| All Name and Symbols | ATF7; ATFA; activating transcription factor 7; MGC57182 |
| Accession Number(s) | NP_001123531.1; NP_006847.1; NP_001123532.1 |
| Peptide Sequence | HVIMTPQSQSAGR, is from C Terminus |
| Human Gene ID(s) | 11016 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all three reported isoforms (NP_001123531.1, NP_006847.1 and NP_001123532.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 35kDa band seen in HeLa cell lysates. Recommended for use at 0.5-2µg/ml. Please note the band size is in agreement with previous experimental data (J Biol Chem 2001 Apr 27;276(17) |
| Immunohistochemistry | In paraffin embedded Human Testis shows staining of select spermatocytes in Human Testis. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Peters CS, Liang X, Li S, Kannan S, Peng Y, Taub R, Diamond RH. ATF-7, a novel bZIP protein, interacts with the PRL-1 protein-tyrosine phosphatase. J Biol Chem 2001 Apr 27;276(17) |
