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Stromal Cell Derived Factor-1 Beta Human Recombinant (CXCL12)
Product Name Stromal Cell Derived Factor-1 Beta Human Recombinant (CXCL12)
Synonyms SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b.
Description Stromal Cell-Derived Factor-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8508 Dalton. The SDF-1b is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P48061
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized SDF-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The CXCL12 protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Greater than 98.0% as determined by
Application
Solubility It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1b in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80ng/ml corresponding to a Specific Activity of 12,500-50,000IU/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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