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Insulin-Like Growth Factor-2 Human Recombinant


Product Name Insulin-Like Growth Factor-2 Human Recombinant
Synonyms Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.
Description Insulin-Like Growth Factor-II Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7505 Dalton. IGF-II is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P01344
Amino Acid SequenceAYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized IGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFII should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized with no additives. Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized IGF2 in sterile 18MO-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a Specific Activity of 1,176,470IU/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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