Goat anti-GRAP Antibody |
| Principle Name | GRAP |
| Official Symbol | GRAP |
| All Name and Symbols | GRAP; GRB2-related adaptor protein; growth factor receptor-bound protein 2-related adaptor protein; MGC64880 |
| Accession Number(s) | NP_006604.1 |
| Peptide Sequence | GFFPRSYVQPVHL, is from C Terminus |
| Human Gene ID(s) | 10750 |
| Non-Human GeneID(s) | 71520 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 26kDa band observed in human thymus lysates (calculated MW of 25.3kDa according to NP_006604.1). Recommended concentration |
| Reference(s) | Feng GS, Ouyang YB, Hu DP, Shi ZQ, Gentz R, Ni J. Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway. J Biol Chem 1996 May 24;271(21) |
