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Interferon-Alpha 2b Human Recombinant, Yeast


Product Name Interferon-Alpha 2b Human Recombinant, Yeast
Synonyms Interferon alpha 2b, IFNA, INFA2, IFN-? 2b, MGC125764, MGC125765.
Description Interferon-alpha 2b Human Recombinant produced in yeast is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of approximately 19 kDa.The Interferon-alpha 2b gene was obtained from human leukocytes.The IFN-a 2b is purified by proprietary chromatographic techniques.
Uniprot Accesion Number Q86UP4
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln.
Source Saccharomyces cerevisiae.
Physical Appearance and Stability Lyophilized glycosilated IFN-a 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity Lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH-7.4. Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized glycosilated IFN alpha 2b in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The specific activity as determined in a viral resistance assay was found to be no less than 300,000,000IU/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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