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Rat Monoclonal anti-mouse R-Spondin-4


Item#:
mAP-0520
Size Price Quantity
50.0 ug $245.00
Catalogue Number mAP-0520
Rat Monoclonal anti-mouse R-Spondin-4
Size 100ug
Immumogen Recombinant Mouse R-Spondin-4
Clone RM0520-16R6
Antibody Isotype Rat IgG2
Preparation This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse R-Spondin-4 (roof plate-specific spondin-4). It is also called cysteine-rich and single thrombospondin domain containing-4 (Cristin 4) and is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R-Spondin family members).
Formulation and Storage The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity This antibody recognizes mouse R-Spondin-4 by direct ELISAs and WBs
Reconstitution Reconstitute the antibody with 500ul sterile PBS and the final concentration is 200ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at <-20 for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.
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