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Rat Monoclonal anti-mouse R-Spondin-3


Item#:
mAP-0519
Size Price Quantity
50.0 ug $245.00
Catalogue Number mAP-0519
Rat Monoclonal anti-mouse R-Spondin-3
Size 100ug
Immumogen Recombinant Mouse R-Spondin-3
Clone RM0519-13R5
Antibody Isotype Rat IgG2
Preparation This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse R-Spondin-3 (roof plate-specific spondin-3). It is also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1) and is an ~31 kDa secreted protein that shares ~40% amino acid (aa) identity with the other three R-Spondin family members.
Formulation and Storage The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity This antibody recognizes mouse R-Spondin-3 by direct ELISAs and WBs. It cross react with human R-Spondin-3 by ELISA and WBs.
Reconstitution Reconstitute the antibody with 500ul sterile PBS and the final concentration is 200ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at <-20 for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.
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D.
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