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Sheep Polyclonal anti-mouse Semaphorin 4C


Item#:
mAP-5426
Size Price Quantity
50.0 ug $530.00
Catalogue Number mAP-5426
Sheep Polyclonal anti-mouse Semaphorin 4C
Size 50ug
Immumogen Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4C.
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified mouse Semaphorin 4C protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity Detects mouse Semaphorin 4C in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human Semaphorin 4C and less than 5% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphorin 4F, and Semaphorin 4G is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B. IHC(Paraffin/Frozen)-(1 to 50-100)
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