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Sheep Polyclonal anti Mouse PU.1 (Spi-1)


Item#:
mAP-5385
Size Price Quantity
50.0 ug $295.00
Catalogue Number mAP-5385
Sheep Polyclonal anti Mouse PU.1 (Spi-1)
Size 50ug
Immumogen E. coli-derived recombinant human PU.1 / Spi-1
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified mouse PU.1 / Spi-1 protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70 C.
Specificity Detects mouse PU.1/Spi-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Spi-1 is observed, and less than 1% cross-reactivity with recombinant mouse Spi-B is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles
Applications
A.WB-(1 to 1000-2000)
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