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Sheep Polyclonal anti Mouse Nogo-B


Item#:
mAP-5318
Size Price Quantity
50.0 ug $295.00
Catalogue Number mAP-5318
Sheep Polyclonal anti Mouse Nogo-B
Size 50ug
Immumogen Mouse myeloma cell line NS0-derived recombinant mouse Nogo-B
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified mouse Nogo-B protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70 C.
Specificity Detects mouse Nogo-B in direct ELISAs and Western blots. In direct ELISAs, approximately 5% crossreactivity with recombinant human (rh) Nogo-B and recombinant rat (rr) Nogo-A (aa 2-172) is observed and less than 1% cross-reactivity with rhNogo-A, rrNogo-A (aa 544-725), and rrNogo-A (aa 1026-1091) is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B. IHC(Paraffin/Frozen)-(1 to 50-100)
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