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Sheep Polyclonal anti Mouse TNF-alpha


Item#:
mAP-5490
Size Price Quantity
50.0 ug $295.00
Catalogue Number mAP-5490
Sheep Polyclonal anti Mouse TNF-alpha
Size 50ug
Immumogen E. coli-derived recombinant human TNF-alpha
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified mouse TNF-alpha protein
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70 C.
Specificity Detects mouse TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF-alpha is observed and less than 1% cross-reactivity with recombinant human TNF-alpha is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B. ICC-(1 to 100-200)
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