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Sheep Polyclonal anti Mouse LIF


Item#:
mAP-5260
Size Price Quantity
50.0 ug $295.00
Catalogue Number mAP-5260
Sheep Polyclonal anti Mouse LIF
Size 50ug
Immumogen E. coli-derived recombinant human LIF
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified mouse LIF protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70 C.
Specificity Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (nonreducing conditions), less than 5% cross-reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross-reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B. N/B-Yes
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