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Sheep Polyclonal anti-mouse Desert Hedgehog (Dhh N-terminus)


Item#:
mAP-5128
Size Price Quantity
50.0 ug $245.00
Catalogue Number mAP-5128
Sheep Polyclonal anti-mouse Desert Hedgehog (Dhh N-terminus)
Size 50ug
Immumogen E. coli-derived recombinant human Desert Hedgehog/Dhh NTerminus
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified mouse Desert Hedgehog/Dhh NTerminus protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity Detects human and mouse Desert Hedgehog/Dhh N-Terminus in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Shh N-terminal peptide (aa 25-198) is observed. Additionally, in direct ELISAs, no cross-reactivity with rmDhh C-terminal peptide (aa 199-396) and rmIhh C-terminal peptide (aa 241-449) is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B. IHC(Paraffin/Frozen)-(1 to 50-100)
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