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Sheep Polyclonal anti-mouse CCL4 (MIP-1 beta)


Item#:
mAP-5072
Size Price Quantity
50.0 ug $245.00
Catalogue Number mAP-5072
Sheep Polyclonal anti-mouse CCL4 (MIP-1 beta)
Size 50ug
Immumogen E. coli-derived recombinant mouse CCL4/MIP-1 beta
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified mouse CCL4/MIP-1 beta protein
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity Detects CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% crossreactivity with recombinant human (rh) MIP-1 beta and recombinant mouse (rm) MIP-1 gamma is observed and approximately 10% cross-reactivity with rmMIP-1 alpha is observed. Neutralizes mouse MIP-1 beta. This antibody will not neutralize the biological activity of rmMIP-1 alpha. In the chemotaxis assay this antibody will partially neutralize rhMIP-1 alpha and rhMIP-1 beta at a 10-30 fold higher IgG concentration
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B. IHC(Paraffin/Frozen)-(1 to 50-100)
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