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Sheep Polyclonal anti Human TNF-alpha

Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5516
Sheep Polyclonal anti Human TNF-alpha
Size 50µg
Immunogen E. coli-derived recombinant human TNF-alpha
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human TNF-alpha protein
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C.
Specificity Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant feline TNF-alpha, recombinant canine TNF-alpha, recombinant rhesus monkey TNF-alpha, and recombinant equine TNF-alpha is observed and approximately 15% cross-reactivity with recombinant cotton rat TNF-alpha, recombinant mouse TNF-alpha, recombinant rat TNF-alpha, and recombinant guinea pig TNF-alpha is observed and less than 5% cross- reactivity with recombinant porcine TNF-alpha and recombinant bovine TNF-alpha is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
A.WB-(1 to 1000-2000)
B.ICC-(1 to 100-200)