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Sheep Polyclonal anti Human Notch-1


Item#:
hAP-5363
Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5363
Sheep Polyclonal anti Human Notch-1
Size 50µg
Immunogen E. coli-derived recombinant human Notch-1
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human Notch-1 protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C.
Specificity Detects the intracellular domain (ICD) of human Notch-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Notch-2 ICD, rhNotch-3 ICD, and rhNotch-4 ICD is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.ICC-(1 to 100-200)
C.FC-Yes
D.ChIP-Yes

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