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Sheep Polyclonal anti Human IL-2


Item#:
hAP-5260
Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5260
Sheep Polyclonal anti Human IL-2
Size 50µg
Immunogen E. coli-derived recombinant human IL-2
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human IL-2 protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C.
Specificity Detects human IL-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% crossreactivity with recombinant rabbit IL-2 is observed, less than 50% cross-reactivity with recombinant canine IL-2, recombinant equine IL-2, recombinant porcine IL-2, recombinant feline IL-2, and recombinant cotton rat IL-2 is observed, and approximately 10% cross-reactivity with recombinant bovine IL-2, recombinant mouse IL-2 and recombinant rat IL-2 is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.ICC-(1 to 100-200)
C.N/B-Yes
D.

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