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Sheep Polyclonal anti Human IL-2 R alpha


Item#:
hAP-5259
Size Price Quantity
50.0 ug $295.00
Catalog Number hAP-5259
Sheep Polyclonal anti Human IL-2 R alpha
Size 50ug
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-2 R alpha
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human IL-2 R alpha protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70 C.
Specificity Detects human IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human (rh) IL-2 sR gamma is observed, less than 5% cross-reactivity with rhIL-6 sR is observed, and less than 1% cross-reactivity with rhIL-1 sRII and rhIL- 2 sR beta is observed. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-2 R alpha is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.N/B-Yes
C.FC-Yes
D.

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