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Sheep Polyclonal anti Human IL-10


Item#:
hAP-5253
Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5253
Sheep Polyclonal anti Human IL-10
Size 50µg
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10.
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human IL-10 protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C.
Specificity Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% crossreactivity with recombinant Epstein-Barr virus IL-10 is observed, approximately 15% cross-reactivity with recombinant mouse IL-10, recombinant feline IL-10, recombinant canine IL-10, recombinant porcine IL-10, and recombinant equine IL-10 is observed and less than 5% cross-reactivity with recombinant rat IL-10, recombinant cotton rat IL-10, and recombinant guinea pig IL-10 is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.IHC(Paraffin/Frozen)-(1 to 50-100)
C.N/B-Yes
D.

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