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Sheep Polyclonal anti Human IL-1 alpha (IL-1F1)

Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5250
Sheep Polyclonal anti Human IL-1 alpha (IL-1F1)
Size 50µg
Immunogen E. coli-derived recombinant human IL-1 alpha/IL-1F1
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human IL-1 alpha/IL-1F1 protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C.
Specificity Detects human IL-1 alpha/IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) IL-1 alpha is observed and less than 5% crossreactivity with recombinant porcine IL-1 alpha , recombinant rat IL-1 alpha , recombinant cotton rat IL- 1 alpha , recombinant human IL-1 beta and rmIL-1 beta is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
A.WB-(1 to 1000-2000)
B.ICC-(1 to 100-200)