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Sheep Polyclonal anti Human IFN-gamma


Item#:
hAP-5245
Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5245
Sheep Polyclonal anti Human IFN-gamma
Size 50µg
Immunogen E. coli-derived recombinant human IFN-gamma
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human IFN-gamma protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C.
Specificity Detects human IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rhesus macaque IFN-gamma is observed and less than 1% crossreactivity with recombinant feline IFN-gamma, recombinant porcine IFN-gamma, recombinant canine IFN-gamma, recombinant rat IFN-gamma, recombinant mouse IFN-gamma, recombinant cotton rat IFN-gamma, recombinant bovine IFN-gamma, and recombinant equine IFN-gamma is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.ICC-(1 to 100-200)
C.N/B-Yes
D.

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