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Sheep Polyclonal anti Human ICAM-1 (CD54)


Item#:
hAP-5235
Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5235
Sheep Polyclonal anti Human ICAM-1 (CD54)
Size 50µg
Immunogen Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1. Extracellular domain
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human ICAM-1/CD54 protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C.
Specificity Selected for its ability to block the adhesion of HSB-2 cells to immobilized recombinant human (rh) ICAM-1. In direct ELISAs, approximately 5% cross-reactivity with rhICAM-3 is observed and less than 2% cross-reactivity with rhICAM-2, rhICAM-4, rhICAM-5, recombinant rat ICAM-1, recombinant mouse ICAM-1, and rhVCAM-1 is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.N/B-Yes

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