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Sheep Polyclonal anti-Human HGF


Item#:
hAP-5225
Size Price Quantity
50.0 ug $295.00
Catalog Number hAP-5225
Sheep Polyclonal anti Human HGF
Size 50ug
Immunogen E. coli-derived recombinant human HGF
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human HGF protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70 C.
Specificity Detects human HGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant canine HGF is observed and less than 5% cross-reactivity with recombinant mouse HGF is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.IHC(Paraffin/Frozen)-(1 to 50-100)
C.Neutralization
D.Detection AB (paired with hAP-0326A)

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